TABLE 1.

Primers used for relative gene expression assessment by real-time reverse transcription-PCR

Gene	Primera	Annealing temp (°C)	Product length (bp)	Reference
TLR3	F, 5′-GGTGTTTCCAGACAATTGGCAAG-3′	60.7	217	53
	R, 5′-TGGAGGTTGTTGTAGGAAAGATCG-3′
TLR7	F, 5′-CCACAGGCTCACCCATACTTC-3′	62.4	129	54
	R, 5′-GGGATGTCCTAGGTGGTGACA-3′
TLR9	F, 5′-ACTGAGCACCCCTGCTTCTA-3′	60.8	198	55
	R, 5′-AGATTAGTCAGCGGCAGGAA-3′
β-Actin	F, 5′-GGCATTGTTACCAACTGGGACGAC-3′	58.6	218	56
	R, 5′-CCAGAGGCATACAGGGACAGCACAG-3′

^aF, forward; R, reverse.