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Transfection with a B19V infectious clone can circumvent the Gb4 block. Transfection of WT and KO cells with pM20. (A and B) mRNA was extracted 1 h or 72 h posttransfection and quantified by RT-qPCR with primers for NS1 mRNA (A) or VP1/VP2 mRNA (B). (C) At 3 days posttransfection, cells were fixed and stained with antibody R9283 against viral capsid proteins. Arrows indicate cells with positive staining. ns, no significant difference.
