TABLE 1.
Virus | No. of mutations to WT-ExoN-DE | Motif I sequencea | No. of reverted lineages by passage 10 at:b
|
|
---|---|---|---|---|
MOI = 0.01 (n = 3) | MOI = 0.5 (n = 3) | |||
ExoN-AA | 4 | GCA…GCT | 0 | 0 |
ExoN-AD | 3 | GCA…GAT | 0 | 0 |
ExoN-EA | 3 | GAA…GCT | 0 | 0 |
ExoN-DA | 2 | GAT…GCT | 0 | 0 |
ExoN-AE | 2 | GCA…GAA | 1c | 3c |
ExoN-ED | 2 | GAA…GAT | 0 | 0 |
ExoN-EE | 1 | GAA…GAA | ND | ND |
ExoN-DD | 1 | GAT…GAT | ND | ND |
WT-ExoN-DE | 0 | GAT…GAA | ND | ND |
Bolded nucleotides must mutate to reach the WT-ExoN-DE genotype.
Recovered viruses were subjected to 10 passages at the indicated MOI. Samples were screened for wild-type revertants by plaque assay, and revertant lineages were sequence confirmed. ND, not done.
By P8 at an MOI of 0.01 and by P2 at an MOI of 0.5.