TABLE 1.
Recovery and passage of intermediate revertants
| Virus | No. of mutations to WT-ExoN-DE | Motif I sequencea | No. of reverted lineages by passage 10 at:b
|
|
|---|---|---|---|---|
| MOI = 0.01 (n = 3) | MOI = 0.5 (n = 3) | |||
| ExoN-AA | 4 | GCA…GCT | 0 | 0 |
| ExoN-AD | 3 | GCA…GAT | 0 | 0 |
| ExoN-EA | 3 | GAA…GCT | 0 | 0 |
| ExoN-DA | 2 | GAT…GCT | 0 | 0 |
| ExoN-AE | 2 | GCA…GAA | 1c | 3c |
| ExoN-ED | 2 | GAA…GAT | 0 | 0 |
| ExoN-EE | 1 | GAA…GAA | ND | ND |
| ExoN-DD | 1 | GAT…GAT | ND | ND |
| WT-ExoN-DE | 0 | GAT…GAA | ND | ND |
a
Bolded nucleotides must mutate to reach the WT-ExoN-DE genotype.
b
Recovered viruses were subjected to 10 passages at the indicated MOI. Samples were screened for wild-type revertants by plaque assay, and revertant lineages were sequence confirmed. ND, not done.
c
By P8 at an MOI of 0.01 and by P2 at an MOI of 0.5.