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. 2019 Sep 30;93(20):e00934-19. doi: 10.1128/JVI.00934-19

FIG 1.

FIG 1

Characterization of DNA and MVA immunogens. (A) Schematic of C.1086 SHIV DNA. (B) Western blot analysis of the SHIV DNA-CD40L construct expressing SIV Gag, SIV CD40L, and HIV C.1086 Env proteins. Sup, supernatant. (C) Flow cytometry displaying surface coexpression of HIV Env and SIV CD40L on SIV Gag-positive transfected 293T cells. (D) Binding of mAb to C.1086-transfected cells. +ve, positive. (E) Electron microcopy images of SHIV DNA-CD40L VLPs budding from the surface of transfected 293T cells. (F) Schematic of C.1086 SHIV MVA constructs. (G) Western blot analysis of the SHIV MVA construct expressing SIV Gag and HIV C.1086 Env proteins. (H) Flow cytometry of Gag-positive cells expressing HIV Env on infected DF1 cells. (I) Binding of mAbs to SHIV MVA-infected cells. (J) Electron microcopy images of MVA-infected DF1 cells expressing SHIV MVA VLPs and MVA particles. (K) Coomassie gels of C.1086 gp140 protein postpurification. CMVIE, cytomegalovirus immediate early promoter.