FIG 2.

LTR and LINE1 retroelements are not suppressed by the transgenic expression of human APOBEC3G in Tlr7^−/− mice. (A to E) Expression of select LTR retrotransposon families and LINE1 families via RT-qPCR using RNA isolated from peripheral blood of C57BL/6N (n = 13), Tlr7^−/− (n = 11), F₃ hA3G⁻ mA3^+/+ Tlr7^−/− (n = 11), F₃ hA3G⁻ mA3^−/− Tlr7^–/– (n = 13), and F₃ hA3G⁺ mA3^−/− Tlr7^−/− (n = 13) mice. Primers amplify the gag or polymerase regions of IAP, MusD, and ETn elements (31) or LINE1 ORFp1. All values are normalized to GAPDH (glyceraldehyde-3-phosphate dehydrogenase) expression. Means and standard deviations are plotted. The adjusted P values in Fig. 2 were calculated for multiple t tests (two-tailed) comparing transposable element expression values of hA3G⁻ mA3^−/− Tlr7^−/− and hA3G⁺ mA3^−/− Tlr7^−/− mice. All adjusted P values were corrected for the five independent hypotheses tested in Fig. 2 using the Holm-Šidák method with an alpha value of 0.05 for the entire family of comparisons. ns, not significant.