Figure 2.

Repeated low-dose NaAsO₂ exposure increased the wound closure speed of HaCat cells. Cells were seeded into 6-well plates. The cell monolayers were scratched using a 200 μl pipette tip to create a wound. Half of the cells were treated with MK2206 (10 μmol/L). DMSO was added into the other half of the cells. Wound width was monitored over time by microscopy and photographed immediately at the time point of 0, 24 and 48 h after MK2206 treatment. (A) Images are the representative photos of three separate experiments. (B) Quantitation of wound width (n = 3). NaAsO₂ exposure increased the wound closure speed, no matter in the presence or absence of MK2206. Although wound closure was inhibited by the treatment of MK2206, NaAsO₂ exposed cells showed higher wound-healing capability than that of the control cells. Significant difference was defined as p less than 0.05. a, vs. the corresponding 0 μM group; b, vs. the corresponding 0.05 μM group; c, vs. the MK2206(-) group of the same NaAsO₂ concentration.