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. 2019 Oct 17;10:305. doi: 10.1186/s13287-019-1403-x

Fig. 1.

Fig. 1

Sample preparation—flow chart. The sample preparation process included (a) hemocytometer analysis, (b) blood smear for each single isolation step, and (c) flow cytometry analysis of samples isolated by density gradient centrifugation and red blood cell lysis. Samples are indicated as follows: whole blood (WB), directly after density gradient centrifugation (DGCun), after density gradient centrifugation, and an additional washing step (DGCw), after the lyse and wash procedure (RBCL)