Fig. 3.

Phylogenetics, gene structure, motif analysis, promoter cis-elements and expression patterns of GhBEL1-like genes. a Phylogenetic relationships of BEL1-like members of upland cotton and A. thaliana. The phylogenetic tree (left panel) was constructed with MEGA 6.0 using the neighbor-joining (NJ) method with 1000 bootstrap replicates. b Gene structure analysis of GhBEL1-like and AtBEL1-like genes. Gene structure maps were drawn with the Gene Structure Display Server 2.0. The scale bar is shown at the bottom. c Motif analysis of GhBEL1-like and AtBEL1-like proteins. All motifs were identified by MEME software (http://meme-suite.org/). The lengths of each motif are shown proportionally. d Cis-element analysis of GhBEL1-like gene promoters. The cis-elements were identified by PlantCARE software for the 1.5 kb upstream from the start codon of GhBEL1-like genes. e The expression patterns of GhBEL1-like genes in various tissues: root, stem, leaf, tours, ovules (− 3 to 3 DPA) and fibers (5 to 25 DPA). The FPKM values of TM-1 RNA-Seq data were used to construct the heatmap