Fig. 2.

VEGF-A, CD34, and D2–40 staining in kidney biopsy. Glomerular VEGF-A was mainly positive in podocytes both, in the control (a) and in the case (b) with no significant difference. Cortical VEGF-A positive staining area was significantly increased in our case (h) than in the control (g). CD34, a marker of endothelium, was positive in glomerular and peritubular capillaries, and arterioles both, in the control (c and i) and in the case (d and j). Both glomerular and cortical CD34 positive staining area were significantly decreased in our case compared to the control. D2–40, a marker of lymphatic vessels, was negative in the glomerulus both in the control (e) and in the case (f). In addition, D2–40 was negative in the targeted cortex area both in the control (k) and in the case (l). Each Fig. 2a, c, or e shows a same glomerulus in the control and each figure (b), (d), or (f) shows a same glomerulus in our case. Each Fig. 2g, i, or k shows a same cortical interstitium area in the control and each Fig. 2h, j, or l shows a same cortical interstitium area in the case. In the cortical interstitium of the case, VEGF-A was mainly positive in the peritubular capillaries but not in the lymphatic vessels. (Original magnification,× 1000)