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. 2019 Oct 11;47(11):e911–e918. doi: 10.1097/CCM.0000000000003977

Figure 3.

Figure 3.

Effects of mechanical ventilation and mechanical cyclic stretch over transient receptor potential vanilloid 4 (TRPV4) phosphorylation. A, Representative Western blots and densitometric quantification showing mechanical ventilation–related increased TRPV4 activation/phosphorylation (serine 824 (serine 824 [pSer824]) in mouse lungs (n = 6). B, Representative Western blots and densitometric quantification showing stretch-induced activation/phosphorylation of TRPV4 at its serine 824 (pSer824) in human epithelial cell line BEAS-2B (n = 5). *p < 0.05 in parametric post hoc test versus the sham group. C, TRPV4 immunohistochemistry of lung sections from lung autopsies obtained from nonventilated (n = 7) and mechanically ventilated patients (n = 10). Immunoreactivity in the ventilated patients is lower compared with the nonventilated patients. The intensity of immunoreaction was semiquantitatively analyzed. **p < 0.05 in Mann-Whitney U test. it-Lido = lidocaine at a dose of 7 mL/kg intratracheally, Lido = lidocaine.