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. 2019 Oct 17;10:304. doi: 10.1186/s13287-019-1414-7

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Changes in anti-inflammatory and vasculogenic potential of MNCs in 5G-culture. a Phase contrast imaging of PBMNCs (at day 0) and E-MNCs (at day 5). The white box areas in the upper images (× 100) were magnified in the lower images (× 200). b Flow cytometric analysis of M1 (CD11b⁺/CD206⁻) macrophages, M2 (CD11b⁺/CD206⁺) macrophages, T lymphocytes (CD3⁺), and B lymphocytes (B220⁺) fractions in PBMNCs (at day 0) and E-MNCs (at day 5). c Percentages of M1 (CD11b⁺/CD206⁻) and M2 (CD11b⁺/CD206⁺) macrophage fractions and the ratios (M1/M2) in PBMNCs and E-MNCs. d mRNA expressions of inflammatory genes (IL-1β and IFN-γ), an anti-inflammatory gene (IL10), and vasculogenic genes (VEGF-A) of PBMNCs (at day 0) and E-MNCs (at day 5) (*p < 0.05). e Counts of EPC-CFUs from pre-cultured PBMNCs and post-cultured PBMNCs (E-MNCs) per dish (1 × 10⁵ cells/dish)