FIG. 2.

RNA-seq transcriptomics of S. aureus COL wild type and the mhqR mutant under MHQ stress. For RNA-seq transcriptomics, S. aureus COL and the mhqR mutant were grown in RPMI1640 medium and treated with 45 μM MHQ stress for 30 min. (A) The gene expression profile of the wild type under MHQ stress is shown as ratio/intensity scatterplot (M/A-plot), which is based on the differential gene expression analysis using DeSeq2 (46). Colored symbols indicate significantly induced (red, orange, yellow, blue, cyan, violet, green) or repressed (dark gray) transcripts (M-value ≥0.6 or ≤−0.6; p ≤ 0.05). Light gray symbols denote transcripts with no fold-changes after MHQ stress (p > 0.05). The TetR, QsrR, MhqR, PerR, CtsR, HrcA, Fur, CsoR, and CstR regulons are most strongly upregulated under MHQ stress. (B) The color-coded heat map displays log2-fold-changes of gene expression between the wild type and the mhqR mutant under control and MHQ. Red and green indicate significantly induced and repressed transcripts (M-value ≥0.6 or ≤ −0.6; p ≤ 0.05) in three biological replicates, respectively. The RNA-seq expression data of all genes under MHQ stress and their regulon classifications are listed in Supplementary Tables S1 and S2. Color images are available online.