FIG. 5.

TSS annotation of the mhqRED mRNA with the 9–9 bp inverted repeat as operator site for the MhqR repressor in S. aureus. (A) The upstream promoter region of the mhqRED operon of S. aureus contains a 9–9 bp palindrome as MhqR operator (denoted with boxes) in position −6 to +12 relative to the TSS that is highly conserved upstream of azoR2, mhqNOP, mhqED, and mhqA of the MhqR regulon in Bacillus subtilis (69). The mapped reads enriched for primary 5′-transcripts of S. aureus USA300 transcriptome under control conditions are displayed for the 5′-end of mhqRED operon using Read Explorer as described in the Experimental Procedures section. The −10 and −35 promoter sequences, the TSS, and the TLS are indicated, and the MhqR operator is marked with arrows. (B) All 9–9 bp MhqR operator sites in front of genes of the MhqR regulons of S. aureus and B. subtilis (69) were aligned (denoted by gray letters), and the MhqR consensus sequence is indicated. TLS, translation start site; TSS, transcription start site.