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. 2019 Oct 17;31(16):1235–1252. doi: 10.1089/ars.2019.7750

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© Verena Nadin Fritsch et al. 2019; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 6. — The MhqR and QsrR regulons confer resistance to MHQ and the antimicrobials pyocyanin and ciprofloxacin. (A, B, D, E, G, and H) For the growth curves, S. aureus COL wild type, mhqR and qsrR mutants, as well as the mhqR complemented strain (mhqR) were grown in RPMI until an OD₅₀₀ of 0.5 and treated with 50 and 150 μM MHQ, 76 μM pyocyanin, and 90.5 μM ciprofloxacin. (C, F, and I) Survival assays were performed by treatment with sublethal and lethal doses and plating 100 μL of serial dilutions onto LB agar plates after 4 h of stress exposure. The survival rates of CFUs for the treated samples were calculated relative to the control, which was set to 100%. The mhqR and qsrR mutants are significantly more resistant to MHQ, pyocyanin, and ciprofloxacin, which could be restored to wild-type levels in the mhqR complemented strain. The results are from four biological replicates. Error bars represent the standard deviation. *p < 0.05; **p < 0.01; ***p < 0.001. CFU, colony-forming unit; LB, Luria–Bertani.