Figure 8.

(A) LFB/H&E staining images of normal spinal cord. Enlargement of framed area in (a) for observation of immunostaining. (B) Image of mESCs colonies on mouse embryonic fibroblast. (C) Image of Oct4/Sox2 immunostaining of mESCs. (D) Image of Olig2+-GFP+ spheres at day 12. (E) Image of Olig2 immunostaining in GFP+ spheres. (F) The percentage of GFP+ cells determined by FACS at day 12. Images of NG2 (G) and PDGFRα (H) immunostaining in GFP+-OPCs at day 14. (I) Image of the GFP+-oligodendrocyte immunostained with MBP. (J) Quantification and comparison of the number of cells expressed NG2, PDGFRα and O4. Reproduced with permission from Sun et al.⁷⁶