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. 2019 Oct 11;9:1056. doi: 10.3389/fonc.2019.01056

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Copyright © 2019 Zmorzyński, Popek-Marciniec, Szudy-Szczyrek, Wojcierowska-Litwin, Korszeń-Pilecka, Chocholska, Styk, Hus and Filip.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electropherograms of studied polymorphisms. (A) Multiplex PCR of GSTT1, GSTM1, and internal control beta-globin genes, Lane 1—Ladder (100 bp); Lanes 2, 3, and 5 contain 480 bp band (GSTT1present); Lane 3 shows 215 bp (GSTM1present); Lane 4—only 325 bp for internal control (GSTT1null/GSTM1null); (B) PCR-RFLP of −308 TNF-α SNPs, Lane 1—Ladder (consists of 34, 67, 110, 147, 190, 242, 331, 404, 489 bp bands); Lanes 2, 3, 5, 6, and 9 contain 87 and 20 bp (GG genotypes); Lanes 4 and 7 show GA genotypes (107, 87, and 20 bp bands), Lane 8 contains 107 bp band (AA genotype); (C) PCR-RFLP of −238 TNF-α SNPs. Lane 1—Ladder (100 bp), Lanes 2–9 show GG genotypes (133 and 19 bp bands); Lane 10 contains 152, 133, and 19 bp band (GA genotype) and Lane 11 shows one band (152 bp)—AA genotype.