Figure 2.

Example of in vitro bortezomib activity (at 4 nM). Note two apoptotic cells, which are stained both AnnCy3 (red) and 6-CF (green) and one necrotic cell (red). For analysis plasmocytes with diameter 9–12 μm were counted. Cells were stained by immunofluorescent technique as described in Material and methods. Total magnification of 1.500×.