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. 2019 Oct 14;12:8339–8353. doi: 10.2147/OTT.S220823

Figure 6.

Figure 6

Expression and role of oncogenic miRNA-92b-3p in esophageal squamous cell carcinoma. (A) Upper panel: The correlation between pathological grade and the expression of potential target genes. Down panel: The correlation between expression of has-mir-92b and the expression of potential target genes. (B) The expression level of miR-92b-3p is significantly up-regulated in ECSS tumor tissue compared to normal adjacent tissue; ***p<0.001; (C) Representative images of positive staining of DSC2 and KLF4 in normal tissues (left) and negative staining of DSC2 and KLF4 in cancer tissues (right). (D) Cell cycle progression was assayed by flow cytometry after transfection with miR-92b mimic for 48h, *p<0.05. (E) The invasive ability of cell lines was performed by transwell assay. (F) Cell migratory ability as determined using wound healing assay. (G, H) immunofluorescence (G) and Western blot (H) analysis was performed for DSC2 and KLF4 expression in ECA-109 cells after transfection with miR-92b-3p mimic or negative control mimic. (I) relative luciferase activity of ECA-109 cells after co-transfection with wild type (wt) or mutant (mut) KLF4(left) and DSC2(right) 3′-UTR reporter genes and miR-92b-3p mimics, *p<0.05.