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. 2019 May 22;25(11):1788–1795. doi: 10.1093/ibd/izz103

FIGURE 3.

FIGURE 3.

Defective proliferation and activation of CARMIL2-deficient T cells upon CD28 costimulation. A, Flow cytometric analysis of CD4+ T cell activation in PBMC from HD, P1, and P2 based on CD25 and CD69 surface expression without (medium) and 2d after stimulation with anti-CD3 coupled beads (α-CD3), anti-CD3 coupled beads plus soluble anti-CD28 (αCD3/CD28), or PMA/ionomycin (P/I) (left panel). B, Dot plots and overlay histogram plots of CFSE dilution and CD25 expression for CD4+ T cells in PBMC from HD, P1, and P2 without (medium) and after stimulation for 5d with anti-CD3 coupled beads (α-CD3), anti-CD3 coupled beads plus soluble anti-CD28 (αCD3/CD28), or PMA/ionomycin (P/I) (left panel). Graphs showing activated CD4+ T cells (A) and proliferated CD4+ T cells and median of CD25 expression (B) in HD (white square), A.I-1 (black rhomb), A.I-2 (black up-pointing triangle), P1 (gray up-pointing triangle), and P2 (gray down-pointing triangle), n = 2 (right panels).