Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Danio rerio) | Tg(pGal4FF:116A) | Characterized in this manuscript | Dr. Koichi Kawakami (NIG, Japan) |
|
| Genetic reagent (Danio rerio) | Tg(pGal4FF:76A) | PMID: 28425439 | Dr. Koichi Kawakami (NIG, Japan) |
|
| Genetic reagent (Danio rerio) | Tg(y333:Gal4) | PMID: 26635538 | Dr. Harold Burgess (NIH) | |
| Genetic reagent (Danio rerio) | Tg(HuC:GCaMP6s) | PMID: 28892088 | Dr. Florian Engert (Harvard) |
|
| Genetic reagent (Danio rerio) | Tg(UAS:GCaMP6s) | PMID: 28425439 | Dr. Koichi Kawakami (NIG, Japan) |
|
| Genetic reagent (Danio rerio) | Tg(UAS:ReaChR-RFP) | Characterized in this manuscript | Dr. Misha Ahrens (Janelia Research Campus) | |
| Genetic reagent (Danio rerio) | Tg(UAS-E1b:NTR-mCherry) | PMID: 17335798 | Available from ZIRC | |
| Genetic reagent (Danio rerio) | Tg(Vglut2a:dsRed) | PMID: 19369545 | ||
| Genetic reagent (Danio rerio) | Tg(Gad1b:loxP-dsRed-loxP-GFP) | PMID: 23946442 | ||
| Genetic reagent (Danio rerio) | Tg(Gad1b:GFP) | PMID: 23946442 | ||
| Genetic reagent (Danio rerio) | Tg(TH2:GCaMP5) | PMID: 26774784 | Dr. Adam Douglass (University of Utah) | |
| Genetic reagent (Danio rerio) | Tg(ETvmat2:GFP) | PMID:18164283 | ||
| Genetic reagent (Danio rerio) | Tg(HCRT:RFP) | PMID: 25725064 | ||
| Antibody | rabbit monoclonal anti-pERK | Cell Signaling | 4370 RRID:AB_2315112 |
IHC (1:500) |
| Antibody | mouse monoclonal anti-ERK | Cell Signaling | 4696 RRID:AB_390780 |
IHC (1:500) |
| Antibody | rabbit polyclonal anti-5-HT | Sigma-Aldrich | S5545 RRID:AB_477522 |
IHC (1:500) |
| Antibody | goat polyclonal anti-5-HT | AbCam | ab66047 RRID:AB_1142794 |
IHC (1:500), 2% BSA in PBS, 0.3% Triton blocking solution) |
| Antibody | goat polyclonal anti-MSH | EMD Millipore | AB5087 RRID:AB_91683 |
IHC (1:500), 2% BSA in PBS, 0.3% Triton blocking solution) |
| Antibody | rabbit polyclonal anti-AGRP | Phoenix Pharmaceuticals | H-003–53 RRID:AB_2313908 |
IHC (1:500) |
| Antibody | rabbit polyclonal anti-MCH | Phoenix Pharmaceuticals | H-070–47 RRID:AB_10013632 |
IHC (1:500) |
| Antibody | rabbit polyclonal anti-CART | Phoenix Pharmaceuticals | 55–102 RRID:AB_2313614 |
IHC (1:500) |
| Antibody | rabbit polyclonal anti-NPY | Immunostar | 22940 RRID:AB_2307354 |
IHC (1:500) |
| Antibody | mouse monoclonal anti-TH | Immunostar | 22941 RRID:AB_1624244 |
IHC (1:500) |
| Chemical compound, drug | DiD’ solid (lipid dye) | Thermo Fisher Scientific | D-7757 | Stock solution (10 mg/ml), working solution (2.5 mg/ml), in ethanol |