Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Antibody	Mouse monoclonal Anti-SLC1A5	Creative Biolabs Inc	TAB-1010CLV	1 ml, 1 mg/ml
Strain, strain background (Homo sapiens)	HEK293S GnTI- cells	ATCC	CRL-3022 RRID: CVCL_A785
Recombinant DNA reagent	pcDNA3.1+	Life Technologies	V79020
Chemical compound, drug	Opti-MEM reduced serum media	Life Technologies	31985062
Strain, strain background (Homo sapiens)	HAP1 SLC1A5 knock out cells	Horizon Discovery	HZGHC005452c002
Chemical compound, drug	Iscove’s modified Dulbecco’s medium	Gibco	12440
Chemical compound, drug	Cell dissociation buffer	Gibco	13151
Chemical compound, drug	Fetal bovine serum	Gibco	16000044
Software, algorithm	MotionCorr2 1.1.0	Zheng et al., 2017	http://msg.ucsf.edu/em/software/motioncor2.html
Software, algorithm	Relion v 2.0	Kimanius et al., 2016	https://www2.mrc-lmb.cam.ac.uk/relion/
Software, algorithm	Phenix 1.14	Adams et al., 2010	http://phenix-online.org/
Software, algorithm	Coot 0.8.9.1	Emsley and Cowtan, 2004	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Software, algorithm	Pymol 2.0	Schrodinger LLC	https://pymol.org/2/
Software, algorithm	Chimera 1.12	Pettersen et al., 2004	https://www.cgl.ucsf.edu/chimera/
Software, algorithm	Gctf	Zhang, 2016	https://www.mrc-lmb.cam.ac.uk/kzhang/
Software, algorithm	cisTem	Grant et al., 2018	https://cistem.org/