Table 1. Cryo-EM 3D reconstruction and refinement of the three Ctf43–CMG complexes.
| Ctf43−CMG1
(EMD-20471) (PDB 6PTJ) |
Ctf43−CMG2
(EMD-20472) (PDB 6PTN) |
Ctf43−CMG3
(EMD-20473) (PDB 6PTO) |
|
|---|---|---|---|
| Data collection and processing | |||
| Magnification | 130,000 | 130,000 | 130,000 |
| Voltage (kV) Electron dose (e-/Å2) |
300 50 |
300 50 |
300 50 |
| Under-focus range (μm) | 1.5–2.5 | 1.5–2.5 | 1.5–2.5 |
| Pixel size (Å) | 1.074 | 1.074 | 1.074 |
| Symmetry imposed | C1 | C1 | C1 |
| Initial particle images (no.) | 759,267 | 759,267 | 759,267 |
| Final particle images (no.) | 200,491 | 53,853 | 53,117 |
| Map resolution (Å) | 3.8 | 5.8 | 7.0 |
| FSC threshold | 0.143 | 0.143 | 0.143 |
| Map resolution range (Å) | 3.5–5.0 | 5.0–8.0 | 5.0–8.0 |
| Refinement | |||
| Initial model used (PDB code) | 3jc5, 4c8h | 3jc5, 4c8h | 3jc5, 4c8h |
| Map sharpening B factor (Å2) | −146 | −135 | −143 |
| Model composition | |||
| Non-hydrogen atoms | 34,366 | 90,831 | 131,141 |
| Protein and DNA residues | 41,92 | 11,221 | 15,710 |
| Ligands | 0 | 0 | 0 |
| R.m.s. deviations | |||
| Bond lengths (Å) | 0.009 | ||
| Bond angels (°) | 1.46 | ||
| Validation | |||
| MolProbity score | 2.05 | ||
| Clashscore | 10.96 | ||
| Poor rotamers (%) Ramachandran plot |
0.63 | ||
| Favored (%) | 91.65 | ||
| Allowed (%) | 8.16 | ||
| Disallowed (%) | 0.19 |