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. Author manuscript; available in PMC: 2019 Oct 18.
Published in final edited form as: J Vis Exp. 2018 Nov 20;(141):10.3791/58593. doi: 10.3791/58593

Figure 2: Arena configuration for trials.

Figure 2:

(A) Open-field testing arena without objects for habituation session. The black arrow indicates a release corner. This corner should be the same relative location in each arena and be consistent for every mouse being tested and for every trial. (B) For the training trial, two different objects are secured to the open field at 6 × 6 cm away from their respective walls. (C) For the OLT, one object is moved to a new location, also 6 × 6 cm away from the walls and not the release corner. (D) For the NORT, the object that was stationary in the OLT is replaced with a novel object while the moved object from the OLT is now the familiar object.