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. Author manuscript; available in PMC: 2020 Apr 8.
Published in final edited form as: Sci Signal. 2019 Oct 8;12(602):eaaw2627. doi: 10.1126/scisignal.aaw2627

Figure 4: POST has 9 transmembrane domains.

Figure 4:

(A) Summary of the predicted number of POST transmembrane domains amongst in silico protein topology prediction programs. The consensus prediction is 10 transmembrane domains. (B to F) The intracellular localization of the N- and C-termini of POST in HEK293 cells was determined by fluorescence protease protection (FPP) assays following trypsin (Trp), digitonin (Dig), and Triton X-100 (Tx). Representative images are shown of fluorescence quenching by trypsin digestion with detergent addition (B; scale bar=5 μm), and representative traces of FPP assays in HEK293 cells expressing C-terminally tagged STIM1 (C), mCherry-POST (D), or POST-GFP (E). Stacked average relative fractions ±SEM of N-terminally (mCherry-POST) and C-terminally (POST-GFP) tagged POST (F). Data are from 3 independent experiments; 17 to 38 cells.