KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-Legionella	Thermo Fisher Scientific	Cat#PA1–7227; RRID: AB_559903
Mouse polyclonal anti-Legionella	Arasaki and Roy, 2010	N/A
Rabbit polyclonal anti-Nogo A+B	Abcam	Cat#ab47085; RRID: AB_881718
Mouse monoclonal anti-Cytochrome C	BD	Cat#556432; RRID: AB_396416
Mouse monoclonal anti-GM130	BD	Cat#610822; RRID: AB_398141
Mouse monoclonal anti-LAMP1 (D4O1S)	Cell Signaling Technology	Cat#15665; RRID: AB_2798750
Rabbit monoclonal anti-FLAG (D6W5B)	Cell Signaling Technology	Cat#14793; RRID: AB_2572291
Mouse monoclonal anti-FLAG M2	Sigma-Aldrich	Cat#F1804; RRID: AB_262044
Mouse monoclonal anti-RABIF (D-12)	Santa Cruz Biotechnology	Cat#sc-390759; RRID: AB_2756826
Rabbit monoclonal anti-Rab10 (D36C4)	Cell Signaling Technology	Cat#8127; RRID: AB_10828219
Rabbit polyclonal anti-Rab1A (C-19)	Santa Cruz Biotechnology	Cat#sc-311; RRID: AB_632290
Mouse monoclonal anti-GAPDH (6C5)	Thermo Fisher Scientific	Cat#AM4300; RRID: AB_2536381
Bacterial and Virus Strains
Legionella pneumophila serogroup 1 strain Lp01	Berger and Isberg, 2003	N/A
Lp01 ΔdotA	Berger and Isberg, 2003	N/A
Lp01 mCherry	Laboratory of Craig Roy	N/A
Lp01 ΔSidC-SdcA + vector	Laboratory of Craig Roy	N/A
Lp01 ΔSidC-SdcA + pSidC	Laboratory of Craig Roy	N/A
Lp01 ΔSidC-SdcA + pSdcA	Laboratory of Craig Roy	N/A
Legionella pneumophila serogroup 1 strain Lp02 rpsL hsdR thyA-	Berger and Isberg, 2003	N/A
Lp02 ΔdotA	Berger and Isberg, 2003	N/A
Lp02 Δ2,3,4,6,7	O’Connor et al., 2011	N/A
Lp02 Δ2,3,6,7	O’Connor et al., 2011	N/A
Lp02 Δ2,3,6	O’Connor et al., 2011	N/A
Lp02 Δ7	O’Connor et al., 2011	N/A
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
Rifampicin	Sigma	R3501
pHrodo Red, succinimidyl ester	Thermo Fisher Scientific	P36600
pHrodo Red E. coli BioParticles Conjugate for Phagocytosis	Thermo Fisher Scientific	P35361
pHrodo Red S. aureus BioParticles Conjugate for Phagocytosis	Thermo Fisher Scientific	A10010
Zymosan A from Saccharomyces cerevisiae	Sigma	Z4250
1 μm amino magnetic polystyrene beads	Spherotech	AM-10–10
Cytochalasin D	Thermo Fisher Scientific	PHZ1063
Isopropyl β-D-thiogalactoside	Sigma	I6758
Hoechst 33342	Thermo Fisher Scientific	H3570
Brefeldin A	LC Laboratories	B-8500
Nocodazole	Sigma	M1404
GFP-Trap_A	Chromotek	Gta-20
Janelia Fluor 646 HaloTag ligand	Laboratory of Luke Lavis	N/A
Critical Commercial Assays
Deposited Data
Raw sequencing data from screens	This paper	SRA: SRP202048
Experimental Models: Cell Lines
Human: U937 cells	ATCC	CRL-1593.2
Human: HeLa cells	ATCC	CCL-2
Human: HEK293 cells	ATCC	CRL-1573
Mouse: Raw 264.7 cells	ATCC	TIB-71
Experimental Models: Organisms/Strains
Oligonucleotides
See Table S5 for sgRNAs used	This paper	N/A
Recombinant DNA
LentiCas9-Blast	Sanjana et al., 2014	Addgene Plasmid #52962
pHR-UCOE-EF1A-Cas9-BFP	Jonathan Weissman	N/A
pMCB306 (pSico-pU6-sgRNA EF1A-Puro-T2A-GFP)	Deans et al., 2016	Addgene Plasmid #89360
pMCB320 (pSico-pU6-sgRNA EF1A-Puro-T2A-mCherry)	Morgens et al., 2017	Addgene Plasmid #89359
pSico-EF1A-Puro-T2A-C1ORF43-GFP	This paper	N/A
pSico-EF1A-G418-T2A-C1ORF43–3xFLAG	This paper	N/A
pSico-EF1A-G418-T2A-C1ORF431–41-3xFLAG	This paper	N/A
pSico-EF1A-Puro-T2A-GFP-RABIF	This paper	N/A
pSico-EF1A-Puro-T2A-GFP-RAB10	This paper	N/A
pSico-EF1A-Puro-T2A-GFP-RAB10 Q68L	This paper	N/A
pSico-EF1A-Puro-T2A-GFP-RAB10 T23N	This paper	N/A
pSico-EF1A-Puro-T2A-GFP-RAB10 K154A	This paper	N/A
pCDNA4/T0-FLAG-SdcA	Horenkamp et al., 2014	N/A
pCDNA4/T0-FLAG-SdcA ΔC	Horenkamp et al., 2014	N/A
Software and Algorithms
casTLE	Morgens et al., 2017	https://bitbucket.org/dmorgens/castle
GOrilla	Eden et al., 2009	http://cbl-gorilla.cs.technion.ac.il/
SAINT	Choi et al., 2011	https://reprint-apms.org/
MaxQuant	Cox and Mann, 2008	https://maxquant.org/
MSstats	Choi et al., 2014	https://bioconductor.org/packages/release/bioc/html/MSstats.html
Fiji	Schindelin et al., 2012	https://fiji.sc/
Prism	Graphpad	https://www.graphpad.com/
Cytoscape	Shannon et al., 2003	https://cytoscape.org/
PyMOL, Version 2.0	Schrödinger	https://pymol.org/2/
Other