Figure 2. AKR1B10 increases DAG levels in breast cancer cells.
DAG and subspecies were measured quantitatively by liquid chromatography-mass spectrum as described in Methods and Materials. (A) Total DAG levels in MCF-7 cells with AKR1B10 expression (left) and BT-20 cells and HCT-8 cells with AKR1B10 silencing (right). (B) Levels of various subspecies of DAG in MCF-7 (upper), BT-20 (lower left) and HCT-8 (lower right) cells. Y axis is in log10. IS, internal standard. *, p<0.05 and **, p<0.01 when compared to vector control.