Table 3.

Summary recommendations for future replication efforts.

Sample Collection Procedures   Sample collected after resting period of 20–45 min with participant in supine position   Sample collected following overnight fast   Rule out acute infection via collection of body temperature and vital signs*

Assaying Procedures   Run all assays in duplicate with coefficients of variation under 10%   Report inter- and intra-assay coefficients   Report assay detection and sensitivity limits

Data Cleaning and Statistical Methods   Report proportion of non-detectable values and patterns of missingness, and number of high out-of-range CRP values, and include all available data (no listwise deletion)   Visualize CRP values to determine outliers   Winsorization for non-detectable and high out-of-range values (three standard deviations)   Correct and transparent reporting of handling of non-normal data, including reporting of kurtosis and skewness statistics   Report results from continuous analyses

Control variables to assess and statistically adjust (as necessary)   Demographic Variables    Age    Sex    Race/ethnicity  Health Variables    Body mass index, waist circumference, or waist-to-hip ratio    Chronic medical conditions or health markers (e.g., cholesterol, triglycerides)*  Substance Variables    Nicotine use (current and former)*    Alcohol use (frequency and amount)*    Caffeine use*  Medication Variables    Antidepressant use (frequency, type & dose)    NSAID use (frequency and dose)*    Statin/anti-hypertensive use (frequency and dose)  Psychosocial Variables    SES and/or education  Additional variables to consider (included in quality score)    Physical activity/exercise    Sleep deprivation

^*Indicates that it is appropriate for the study to exclude based on this variable or to exclude based on acute use.