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. Author manuscript; available in PMC: 2019 Oct 18.
Published in final edited form as: Cold Spring Harb Protoc. 2011 Apr 1;2011(4):pdb.prot5595. doi: 10.1101/pdb.prot5595

FIGURE 2.

FIGURE 2.

Example of a commercial heater box. Static culture system for time-lapse imaging of postimplantation mouse embryos. The microscope and static culture are housed in a temperature- (37°C) and CO2-regulated Plexiglas chamber. The gas mixture for the culture is humidified using a bubbler, and an inlet to the heated stage allows for ample gas exchange during static culture.