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. Author manuscript; available in PMC: 2019 Oct 18.
Published in final edited form as: Metab Eng. 2019 Apr 25;54:222–231. doi: 10.1016/j.ymben.2019.04.010

Fig. 5. Comparison of metabolite pool size between Synechococcus PCC 7002 control and ΔccmKLMN.

Fig. 5.

(A) Relative pool size comparisons between the two strains. A y-axis of 1 represents equal metabolite concentrations while a y-axis > 1 indicates the factor of pool size in ΔccmKLMN greater than PCC 7002 control. The relative pool size was normalized using a13C-labeled internal standard and cell density. (B) Succinate and malate in the culture supernatant were detected by LC-MS, and the peak intensity (counts per second, cps) was proportional to their concentrations. Exact succinate concentration was further quantified using an enzyme kit (Table S1).