Micropatterning of E-selectin and aEpCAM-dendrimers for combining the effects of rolling and multivalent binding under flow. (a) Schematic illustration of the surface marker-dependent cell capture using aPSA, aHER-2, and aEpCAM. (b) The capture patterns of the three cell lines labeled with three different fluorescent colors: green for MDA-PCa-2b, blue for MDA-MB-361, and red for MCF-7. The lower capture efficiency of MCF-7 cells for aHER-2 due to low HER-2 expression of MCF-7 cells were presented as a dotted, faded circle in the schematic illustration (a). (c) A combination of dendrimers and E-selectin (a cell rolling inducing agent), along with multiple antibodies achieved highly sensitive differential detection of tumor cells (up to 82%, Error bars: standard error (n=4)). (Copyright American Chemical Society, Ref. 28)