Figure 1. Hypoxia modifies the pancreatic cancer cell proteome under both serum-replete (S) and serum-free conditions (SF).

(A) PDA cell proliferation curve during culture in S and SF conditions confirming growth restriction during hypoxia. (B) Western blot of HIF-1α protein and RT-PCR assessment of fold change (Hx/Nx) in hypoxia markers NDRG1 and HK2. (C) the number of proteins identified in PDA cells after culture under the different conditions indicated. (D) Analysis of protein distribution against log₂ H/L ratio for the S and SF culture conditions during normoxia/hypoxia revealed that the majority of these proteins are downregulated during hypoxia. Nx, normoxia; Hx, hypoxia; S, serum/serum replete; SF, serum-free; H/L, heavy/light chain ratio.