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. 2019 Oct 15;10(57):5894–5905. doi: 10.18632/oncotarget.27185

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Copyright: © 2019 Ribaux et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A) Anti-PEDF antibodies (light grey bars) or control IgG (dark grey bars) were added in culture medium of cells treated with 100 nM Ptx. FBS: fetal bovine serum. (B) Western blot analysis of PEDF in FBS and ascites (C) Western blot analysis of PEDF in ascites, PEDF-depleted ascites (A-PEDF) and purified PEDF (pPEDF). Left panel: Ponceau red staining of membrane; Right panel: PEDF immunoblotting. (D) Effect of ascites-PEDF on viability of cells treated with 100 nM Ptx (for 48h) compared to FBS-PEDF. (E) Effect of PEDF depletion from ascites on Ptx treated SKOV3-derived tumor growth. ^** p < 0.01.