Table 1.
Sequence Of Primers Used In This Study
| (a) List And Sequences Of RT-PCR Primers | |||||
|---|---|---|---|---|---|
| Gene | F/R | Sequence (5ʹ > 3ʹ) | Melting Temperature (°C) | Product Size (bp) | |
| IL-1β | F R |
AGCTACGAATCTCCGACCAC CGTTATCCCATGTGTCGAAGAA |
61.1 60.1 |
186 | |
| HCA1 | F R |
CTCATTGTGGCCTTTGTGCT GAATGTCCCCAAAAGCCCAG |
59.0 59.1 |
196 | |
| HCA2 | F R |
GCTGTGTGTTCCGAGATGAC CCAAACTTCCAGTCCCAACG |
58.9 59.0 |
235 | |
| HCA3 | F R |
TGCCAAGATCAAGAGAGCCA GAAGTTGGGAAAGGATGGGC |
59.0 58.8 |
244 | |
| GSDMD | F R |
GTGTGTCAACCTGTCTATCAAGG CATGGCATCGTAGAAGTGGAAG |
60.5 60.7 |
94 | |
| (b) List and sequences of primers used in MSP | |||||
| Gene | F/R | M/U | Sequence (5ʹ > 3ʹ) | Melting Temperature (°C) | Product size (bp) |
| GSDMD | F R |
M | ATTTTGTATACGTAGTAGAAGGGACG TTACGAAAATCGACGAAACG |
58.3 58.8 |
98 |
| GSDMD | F R |
U | TGTATATGTAGTAGAAGGGATGTGG AAAAATCAACAAAACACCCT |
56.5 53.8 |
90 |
Abbreviations: F, forward; R, reverse; M, methylated; U, unmethylated.