Figure 3.

SD-induced consolidation memory deficit was not attributed to its effect on circadian proBDNF expression. A, Conditioned rats were subjected to 4-h SD with or without proBDNF infusion ZT0. At the ZT4 of the following day, to tested fear memory, rats were returned to the same contextual chambers and presented with as few as one tone. B, SD impaired memory consolidation of conditioned fear, while injection of cleavage-resistant proBDNF into the vHPC did not rescue the defect; **p < 0.01 versus CON; n = 7 per group. Immunohistochemical detection of proBDNF in vHPC, PFC, striatum, cerebellum, diencephalon and amygdala of SD and control rats. Digital light micrographs (20×) of proBDNF-positive cells (C) and sum of IOD comparison (D); *p < 0.05 versus CON; n = 5 per group.