Table 1.
Viruses used in this study and the analytical specificity results of real‐time RT‐PCR and insulated isothermal RT‐PCR
| Virus lineage | Isolate name | Subtype | RRT‐PCR (C t value, 0:no C t) | iiRT‐PCR (1 pos, 0 neg) | |||
|---|---|---|---|---|---|---|---|
| M InfA | H7 CODA | H7 CNIC | H7 | N9 | |||
| Index virus 2013 | A/Anhui/1/2013 | H7N9 | 18.1 | 20.5 | 21.5 | 1 | 1 |
| H7N9 viruses of Yangtze River lineage 2015‐2017 (n = 11) | A/Ck/ST/2969/2017 | H7N9 | 16.5 | 18.2 | 19.2 | 1 | 1 |
| A/Ck/ST/881/2017 | H7N9 | 19.9 | 19.2 | 19.9 | 1 | 1 | |
| A/Ck/ST/586/2017 | H7N9 | 15.4 | 17.4 | 17.9 | 1 | 1 | |
| A/Ck/NJ/578/2017 | H7N9 | 20.0 | 20.2 | 21.9 | 1 | 1 | |
| A/Ck/HZ/656/2017 | H7N9 | 15.5 | 16.6 | 21.0 | 1 | 1 | |
| A/Ck/HZ/123/2017 | H7N9 | 15.9 | 18.5 | 22.8 | 1 | 1 | |
| A/Env/NJ/739/2017 | H7N9 | 11.9 | 15.0 | 21.0 | 1 | 1 | |
| A/Ck/ST/750/2017 | H7N9 | 20.0 | 18.7 | 21.6 | 1 | 1 | |
| A/SCk/ST/1059/2017 | H7N9 | 13.6 | 16.4 | 20.3 | 1 | 1 | |
| A/Ck/ST/1497/2017 | H7N9 | 13.6 | 16.3 | 17.2 | 1 | 1 | |
| A/Ck/ST/2439/2017 | H7N9 | 19.4 | 21.8 | 25.9 | 1 | 1 | |
| H7N9 viruses of Pearl River lineage 2015‐2017 (n = 11) | A/Ck/GZ/1273/2017 | H7N9 | 18.3 | 19.3 | 21.6 | 1 | 1 |
| A/Ck/DG/3082/2016 | H7N9 | 17.1 | 18.3 | 20.3 | 1 | 1 | |
| A/Ck/DG/3090/2016 | H7N9 | 13.3 | 15.4 | 26.1 | 1 | 1 | |
| A/Ck/DG/3097/2016 | H7N9 | 18.9 | 16.7 | 27.4 | 1 | 1 | |
| A/Ck/DG/A3/2017 | H7N9 | 16.7 | 19.2 | 30.8 | 1 | 1 | |
| A/Ck/DG/A12/2017 | H7N9 | 13.5 | 16.5 | 28.2 | 1 | 1 | |
| A/Ck/DG/A101/2017 | H7N9 | 15.8 | 18.9 | 30.6 | 1 | 1 | |
| A/Ck/GZ/361/2017 | H7N9 | 15.8 | 17.1 | 19.5 | 1 | 1 | |
| A/Ck/GZ/372/2017 | H7N9 | 16.6 | 16.3 | 19.1 | 1 | 1 | |
| A/Ck/GZ/484/2017 | H7N9 | 21.0 | 20.9 | 21.8 | 1 | 1 | |
| A/Ck/GZ/1183/2017 | H7N9 | 18.4 | 20.1 | 22.3 | 1 | 1 | |
| Highly pathogenic H7N9 viruses of 2017 (n = 5) | A/ GD/17SF003/2017 | H7N9 | 18.2 | 18.5 | 22.3 | 1 | 1 |
| A/Ck/GZ/418/2017 | H7N9 | 19.6 | 21.6 | 22.7 | 1 | 1 | |
| A/Ck/GZ/430/2017 | H7N9 | 15.3 | 17.1 | 20.7 | 1 | 1 | |
| A/Ck/GZ/837/2017 | H7N9 | 13.4 | 16.4 | 17.9 | 1 | 1 | |
| A/Ck/GZ/1494/2017 | H7N9 | 11.8 | 15.2 | 17.3 | 1 | 1 | |
| H7 viruses of Eurasian lineage but not in the cluster of recent Chinese H7N9 (n = 7) | A/Dk/Thai/CU7280C/2010 | H7N6 | 22.4 | 19.7 | 26.7 | 1 | 0 |
| A/Dk/Thai/CU7279T/2010 | H7N6 | 18.9 | 18.2 | 22.9 | 1 | 0 | |
| A/Dk/Thai/CU9744C/2010 | H7N4 | 25.1 | 20.6 | 35.9 | 1 | 0 | |
| A/Dk/Thai/CU10507T/2011 | H7N4 | 17.6 | 17.6 | 0 | 1 | 0 | |
| A/Dk/JX/1760/2003 | H7N7 | 20.7 | 17.3 | 27.4 | 1 | 0 | |
| A/Dk/VN/CT/LBM25/2013 | H7 | 22.5 | 21.0 | 0 | 1 | 0 | |
| A/Dk/VN/AG/LBM441/2013 | H7 | 23.3 | 20.1 | 0 | 1 | 0 | |
| Avian influenza virus subtype H1‐H6, H8‐H13 (n = 15) | A/WB/ST/520/2000 | H1N9 | 17.4 | 0 | 0 | 0 | 1 |
| A/DK/ST/1090/2000 | H2N3 | 17.6 | 0 | 0 | 0 | 0 | |
| A/Dk/ST/1283/2001 | H3N8 | 16.1 | 0 | 0 | 0 | 0 | |
| A/Dk/ST/472/2003 | H4N6 | 18.2 | 0 | 0 | 0 | 0 | |
| A/Ck/HK/Yu22/2002 | H5N1 | 13.9 | 0 | 0 | 0 | 0 | |
| A/Gs/GY/3375/2014 | H5N6 | 14.1 | 0 | 38.1 | 0 | 0 | |
| A/Dk/ST/22636/2008 | H6N2 | 18.2 | 0 | 0 | 0 | 0 | |
| A/Dk/ZZ/1883/2017 | H6N6 | 19.6 | 0 | 0 | 0 | 0 | |
| A/WB/HK/MP2411/2004 | H8N4 | 16.5 | 0 | 0 | 0 | 0 | |
| A/Quail/HK/G1/1997 | H9N2 | 16.9 | 0 | 0 | 0 | 0 | |
| A/Ck//WZ/598/2013 | H9N2 | 15.3 | 0 | 0 | 0 | 0 | |
| A/Dk/ST/1796/2001 | H10N8 | 19.6 | 0 | 0 | 0 | 0 | |
| A/Dk/ST/4253/2003 | H11N3 | 14.8 | 0 | 0 | 0 | 0 | |
| A/Mallard/VN/66MD/2004 | H12N9 | 17.2 | 0 | 0 | 0 | 1 | |
| A/Gull/MD/704/1977 | H13N6 | 17.5 | 0 | 0 | 0 | 0 | |
| Swine influenza virus H1 and H3 (n = 3) | A/Swine/HK/1110/2006 | H1N2 | 15.4 | 0 | 38.1 | 0 | 0 |
| A/Swine/GD/NS2788/2011 | H1N1 | 12.8 | 0 | 0 | 0 | 0 | |
| A/Swine/HK/NS2811/2011 | H3N2 | 14.6 | 0 | 0 | 0 | 0 | |
| Human influenza virus H1 and H3 (n = 4) | A/California/07/2009 | H1N1 | 17.3 | 0 | 0 | 0 | 0 |
| A/Michigan/45/2015 | H1N1 | 21.6 | 0 | 0 | 0 | 0 | |
| A/HK/1/1968 | H3N2 | 16.7 | 0 | 0 | 0 | 0 | |
| A/HK/4801/2014 | H3N2 | 16.9 | 0 | 0 | 0 | 0 | |
| Newcastle disease virus (n = 1) | NCVD/A372/2012 | NDV | 0 | 0 | 0 | 0 | 0 |
| Infectious bursal disease virus (n = 1) | NCVD/A102/2009 | IBDV | 0 | 0 | 0 | 0 | 0 |