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. 2019 Sep 30;116(42):21207–21212. doi: 10.1073/pnas.1905721116

Fig. 2.

Fig. 2.

Deletion of Grin1 exon 5 prolongs the time course of NMDAR-EPSCs at the thalamic relay synapse. (A) Generation of the mouse strain carrying targeted deletion of Grin1 exon 5 (Grin1ΔEx5). The Grin1 floxed Neo strain was generated by ES targeting in a C57BL/6J background. Exon 5 of Grin1 and the Pgk-Neo cassette was then excised using a germ line Cre driver. (B) RT-PCR for Grin1 exon 5, total Grin1, Grin2a, Grin2b, and the housekeeping gene Pgk using thalamic tissues from WT and Grin1ΔEx5/ΔEx5 mice. (C) Examples of NMDAR-EPSCs recorded at the lemniscal synapse from WT and Grin1ΔEx5/ΔEx5 mice at P7 and P16. EPSCs are normalized to the peak. (D) Weighted decay constants (τw) of NMDAR-EPSCs of WT (black), heterozygous (blue), and homozygous (red) mutant mice at various ages. Data are mean ± SEM from 12 to 28 cells in 3 or 4 mice. ***P < 0.0005, Mann–Whitney U or Kruskal-Wallis test.