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. 2019 Sep 5;116(42):21191–21197. doi: 10.1073/pnas.1903763116

Fig. 5.

Fig. 5.

Function of Rac1/SCAR/Dia forgetting pathway in the MB γ-neurons. (A) Expression patterns of 5-HT1B-GAL4. Besides the strong expression in the MB γ-neurons, the Gal4 also labels neurons outside the MB, including the ellipsoid body in the central complex (arrow). MB-Gal80 suppressed the expression of 5-HT1B-GAL4 in the MB neurons specifically. Green, mCD8-GFP reporter; magenta, nc82. (Scale bar, 50 μm.) (B) Memory retention curves. Conditional expression of Rac1-DN in the adult MB γ-neurons with the 5-HT1B-Gal4 driver was sufficient to slow down memory decay, and the effect was blocked by MB-Gal80 (24 h). n = 6 to 10. (C) Consistent with Rac1-DN, RNAi knockdown of SCAR/WAVE complex members in the MB γ-neurons led to higher memory retention at 6 h. n = 8 to 10. (D) Knockdown of Dia in the adult MB γ-neurons resulted in higher memory retention at 6 h. n = 6. Data are means ± SEM. *P < 0.05. n.s., nonsignificant.