Fig. 3.

Genome-wide editing specificity of WT and combinatorial mutant SaCas9 variants at selected targets using GUIDE-seq. (A) Percentage of edited reads detected by GUIDE-seq at the on-target site (green) and off-target sites (ordered by number of mismatches) among total edited reads by each SaCas9 (Top), and numbers of genome-wide off-target sites (Bottom). (B) Venn diagram comparing off-target sites for WT, the best-performing double (R245A/N413A) and triple (R245A/N413A/N419A) mutant, and SaCas9-HF at the target VEGFA_8.