Fig. 4.
Aloperine is a potent and selective activator of KCNQ5 channels. All error bars indicate SEM. (A) Mean TEVC current traces showing effects of aloperine (100 µM) on KCNQ2/3 and homomeric KCNQ2, KCNQ3*, and KCNQ4 expressed in Xenopus oocytes (n = 5 to 10). (B) Mean tail current (Left) and mean normalized tail currents (G/Gmax; Right) versus prepulse voltage relationships for the traces as in A (n = 5 to 10). (C) Effects of aloperine (100 µM) on resting membrane potential (EM) of unclamped oocytes expressing channels as in A (n = 5 to 10). n.s., not statistically significant (P > 0.05). (D) Voltage dependence of effects of aloperine (100 µM) on channels indicated (from traces as in A; n = 5 to 10). (E) Aloperine dose response calculated from fold increase in current at −60 mV for channels as indicated (n = 5 to 10).