Fig. 3.

Evolutionary comparison of Arabidopsis and human Cys-SOH sites. (A) Overrepresentation motifs of Cys-SOH sequence windows (−6 to +6 residues) of human (20) and Arabidopsis BTD-labeled sites constructed by iceLogo (39). Basic residues Lys, Arg, and His are in blue, and Cys is in red. (B) Overview of Arabidopsis Cys-SOH alignment scenarios and their occurrence. (C) Examples of Arabidopsis (green)–human (blue) protein sequence alignments with exact alignments of S-sulfenylation events (red, with protein position indicated). Ratios (R_H2O2/Ctrl) were derived from this study (Dataset S1), with the number of biological replicates indicated between parentheses, and as reported for human RKO cells treated with H₂O₂ (23).