Fig. 3.

Production of diap1*-dsRNA by C. glutamicum in batch fermentation. a Growth of C. glutamicum 2256LΔrnc harboring pVC7H2 as a control or pVH2-HvIap-1. Average values and standard deviations (SDs) for three independent experiments are shown, although the variation range is too small to distinguish the SDs on the graph. b PAGE analysis of total RNA prepared from each C. glutamicum strain during the fermentation. Lane M shows dsRNA marker, and each culture time (h) is indicated at the top of the gel. The arrow and asterisks indicate the positions of diap1*-dsRNA and its possible degradation products, respectively. The result presents one representative diap1*-dsRNA production experiment in a jar fermentor