Fig. 6.

Effect of feeding H. vigintioctopunctata diap1*-dsRNA-producing C. glutamicum cells treated with alcohol or by heating on leaf consumption activity. After feeding the sample, the areas of leaf consumed during hours 0–24 (gray bars) and subsequently during hours 24–48 (black bars) were measured. Sample 1, larvae fed water as a control; 2, 4, 6, larvae fed C. glutamicum 2256LΔrnc cells harboring pVC7H2 vector; 3, 5, 7, larvae fed C. glutamicum 2256LΔrnc cells harboring pVH2-HvIap-1. The data are presented as the mean ± SD. For each group, 4–5 larvae were used. Asterisks indicate significant differences (P < 0.05) from corresponding values for the strain harboring only vector