Fig. 7.

Damage to potato leaves by larvae of H. vigintioctopunctata and its suppression by feeding sterilized microbial cells containing diap1*-dsRNA to the pest. C. glutamicum cells harboring pVC7H2 vector (a) or pVH2-HvIap1 (b) were treated with 80% ethanol for sterilization and then fed to H. vigintioctopunctata larvae. The status of potato leaves eaten between 24 and 48 h after the feeding of sterilized cells to the larvae is shown. A typical result from four to five leaves from each experimental group is presented. Scale bar, 1 cm