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. 2019 Oct 18;10:4769. doi: 10.1038/s41467-019-12741-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Stratification of pumas based on the geographic population. a Principal component analysis of 166,037 sites separates the sampled pumas based on population. The first component primarily separates South and North American pumas, while the second component distinguishes the variation within North America. All California pumas (Santa Cruz and Santa Monica) cluster closely. b TreeMix⁶² analysis, using the African cheetah as the outgroup, indicates the best tree separates pumas based on population and includes one migration event (weight = 0.453911) from the branch of South American diversity into the admixed Everglades puma (EVG21). c The mean of ten permuted matrices of STRUCTURE⁶³ analysis for each of K = 2 through 4, performed using CLUMPP⁶⁴. Both delta K and L(K) values indicated that K = 3 was the best K (Supplementary Fig. 10)⁶⁵.