Fig. 4.

Heterozygosity and runs of homozygosity. a Sliding window heterozygosity (black dots) and called ROH (colored boxes) across a single scaffold for three pumas from three different populations (Big Cypress, Yellowstone, and Brazil). Plots for all pumas are provided as Supplementary Fig. 12. b Average genome-wide heterozygosity versus the proportion of the genome in ROH for the ten pumas sequenced. c Distribution of lengths of ROH. The length in Mb is indicated, as is the associated expected number of generations since the individual’s maternal and paternal lineages shared a common ancestor. d Heat map showing the percent of the genomes that are in ROH that are shared IBD between pairs of pumas (Supplementary Table 6).