Fig. 1.

Identification of DskGAL4 neurons that inhibit male courtship. a Experimental design of screening for courtship-inhibiting neurons. b Identification of DskGAL4 and CrzGAL4 that inhibit male courtship when driving UAS-dTrpA1 at 30 °C (red circle) compared to permissive temperature 22 °C (blue square). n = 12 for sNPF-GAL4 and Lk-GAL4, n = 18 for Burs-GAL4, Dh31-GAL4 and Proc-GAL4, n = 20 for SIFaGAL4s, n = 24 for others. ***p < 0.001, Mann–Whitney U test. c Thermogenetic activation of Dsk neurons labeled by another DskGAL4 (attP2) severely inhibits male courtship. n = 24 for each. p < 0.001, Kruskal–Wallis test. ***p < 0.001 and p > 0.1 (n.s.), post hoc Dunn’s multiple comparisons test. d Optogenetic activation of DskGAL4 (attP2) neurons abolishes male courtship. n = 24 for each. p < 0.001, Kruskal–Wallis test. **p < 0.01 and ***p < 0.001, post hoc Dunn’s multiple comparisons test. e Optogenetic activation of DskGAL4 neurons rapidly inhibits male courtship. n = 24 for each. f Courtship inhibition by optogenetic activation of DskGAL4 neurons lasts for more than 10 min after lights off. n = 24. n.s. not significant. Error bars indicate SEM. Source data are provided as a Source Data file