Fig. 2.

Four pairs of Dsk- and fru^M-expressing neurons inhibit male courtship. a Expression pattern of DskGAL4 in the central nervous system revealed by anti-GFP (left) and anti-DSK (middle). Representative of eight male flies. Scale bars, 50 μm. b Two pairs of MP1 and two pairs of MP3 neurons are co-labeled by fru^LexA driving LexAop-RedStinger (magenta) and DskGAL4 driving UAS-Stinger-GFP (green). Representative of five male brains. Scale bars, 50 μm and 20 μm (zoom-in). c Intersectional strategy to label and manipulate Dsk and fru^M co-expressing MP1 and MP3 neurons. Representative of 6 male brains. Scale bars, 50 μm. d Optogenetic activation of intersectional neurons between DskFlp and fru^GAL4 abolishes male courtship. Red square indicates test in red light, and gray circle indicates test in dark. n = 24 for each. p < 0.001, Kruskal–Wallis test. ***p < 0.001 and p > 0.99 (n.s.), post hoc Dunn’s multiple comparisons test. Error bars indicate SEM. Source data are provided as a Source Data file