FIGURE 2.

Database and experimental mapping of the three major transcript isoforms of PTBP1 mRNA. (A) Transcription start sites (TSS) determined by CAGE mapping in the FANTOM5 database (Riken Center for Integrative Medical Sciences [IMS]) (Noguchi et al. 2017), along with hg38 chromosome location, showing the last three digits of the chromosomal coordinates for the PTBP1 gene. (B) Sites of PTBP1 mRNA interaction with eIF3 mapped by photoactivatable RNA crosslinking and immunoprecipitation (PAR-CLIP) (Lee et al. 2015), by eIF3 subunit, indicated to the right. Coordinates of clusters are given in Table 2. (C) Annotated Ensembl transcripts for PTBP1, including the 5′-UTR and the beginning of the CDS, as indicated by the last four digits of the Ensembl tag (i.e., ENST0000****). (D) Experimentally determined 5′-UTR elements in PTBP1 mRNAs determined by RLM-RACE. In all panels, the transcripts are vertically aligned with the chromosomal coordinates in panel A.