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. 2019 Oct;25(10):1298–1310. doi: 10.1261/rna.070425.119

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© 2019 Maudlin and Beggs; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 3. — Depletion of Spt5 affects pre-mRNA splicing. (A) Cartoon showing the RT-qPCR amplicons for splicing analysis for an average gene. These detect pre-mRNA (5′SS or BP and 3′SS), lariat (excised intron or intron–exon 2), exon 2 (ex 2), and mRNA. (B) RT-qPCR analysis of the intron-containing genes ACT1, RPS13, and ECM33 after depletion of Spt5-AID*, normalized to the SCR1 RNAPIII transcript and time zero (without auxin and β-estradiol addition). Mean of three biological replicates. Error bars, standard error of the mean. Asterisks show the statistical significance (Student's unpaired t-test). (*) P < 0.05, (**) P < 0.01, and (***) P < 0.001. Not significant, P > 0.05. Gray crosses indicate the individual replicate values.