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. 2019 Sep;25(9):1211–1217. doi: 10.1261/rna.070177.118

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© 2019 Amini Chermahini et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 2. — RNAscope detects endogenous DUX4 mRNA in 15A FSHD myotubes. FSHD 15A myotubes demonstrated higher amounts of DUX4 mRNA compared to non-FSHD 15V myotubes, as determined by RNAscope staining. Arrows indicate brown punctate signal. (A) Negative control stain. (B) 15V cell line stained with the housekeeping gene PPIB served as a positive control for the assay. (C) DUX4 expression in FSHD 15A myotubes was reduced or absent in (D) 15A cells transfected with U6.mi405 microRNAs. (E) Very weak or absent signal was present in the unaffected 15V cell line alone and in (F) 15V transfected with U6.mi405 plasmid.